Voyager DP-263-S Guía de usuario descargar pdf (Pagina 450)

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Chapter 7 PSD Analysis

7-46 PerSeptive Biosystems

Acquiring

NOTE: In this exercise, you will observe the impact of the

Grid Voltage% on the precursor ion at 1,297 Da and the

fragment ion at 1,181 Da. At the laser intensity you

optimized for the fragment ion, the precursor ion will be

saturated. To allow you to observe precursor and fragment

ions in the spectrum, change the Vertical Scale setting in

the .BIC file (Digitizer/Mode dialog box) to 1,000 mV

full scale.

1. Enable the Precursor Ion Selector if it is disabled.

2. Acquire a spectrum to make sure the precursor is not

saturated.

Hint: If the precursor ion peak is still saturated but the

peak valleys are noticeable, you can estimate the

resolution at the +2 isotopic peak.

3. Make a note of the resolution of the fragment ions and the

precursor ion.

NOTE: You may need to switch between Vertical Scale

settings of 1,000 mV and 200 mV and reacquire spectra

to see the resolution on the precursor and fragment

peaks.

4. From the Acquisition menu, select Stop Experiment, to

allow you to change the Grid Voltage in the Instrument

Settings control page.

5. Decrease the Grid Voltage% value in 5 percent

increments and observe the spectrum. For example, if the

setting in the .BIC file is 80%, decrease to 75%, then 70%.

At lower Grid Voltage% settings:

• Lower mass ion resolution degrades

• Higher mass ion resolution improves

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