Voyager DP-263-S Guía de usuario descargar pdf (Pagina 162)

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Chapter 3 Preparing Samples

3-32 PerSeptive Biosystems

Do not touch the tip of the pipette to the sample spot.

Make sure the sample is evenly applied to the spot.

NOTE: Some organic solvents such as methanol,

acetone, and THF spread very easily on metal

surfaces. If the sample contains these solvents, try to

load smaller volumes (0.5 µl or less).

NOTE: Try to place the sample in the center of the

sample position. If the sample is not in the center, the

laser position shown on the Sample View (see

Figure 4-9 on page 4-31) may not be centered on the

sample.

3. If you are using DHB matrix for neutral carbohydrates, dry

the sample plate quickly under vacuum.

If you are using other matrices, allow the sample plate

to dry for at least five minutes. Preparations with high

water or salt content may require longer to dry.

4. Visually examine the sample spots to make sure they are

dry.

NOTE: If you load the sample plate into the Voyager

Biospectrometry Workstation before the plate is dry, the

pressure in the sample chamber rises, and a “TC2

pressure too high” error code may be displayed in the

Control window. Wait a few minutes for the chamber to

reach pressure.

5. Place a protective cover over the plate until you are ready

to load the plate. Do not allow the cover to touch the

surface of the plate.

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