Voyager DP-263-S Guía de usuario descargar pdf (Pagina 310)

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Chapter 5 Optimizing Instrument Settings

5-80 PerSeptive Biosystems

When to use

Turn on Low Mass Gate when:

• Analyzing masses greater than 2,000–3,000 Da

• Matrix signal is more intense than the sample signal

Optimum setting

for starting mass

You will need to experiment with the optimum setting for the

starting mass (threshold) for Low Mass Gate. As a general

guideline:

• If you are analyzing peptides in the 400 to 2,000 Da

range, set the Low Mass Gate ~350 Da

• If you are analyzing peptides in the 2,000 to 8,000 Da

range, set the Low Mass Gate ~500 Da

• If you are analyzing samples in the 10,000 to 30,000 Da

range, set the Low Mass Gate ~3,000 Da

When not to use

Do not use Low Mass Gate if:

• You are analyzing peaks in the same mass range as

matrix peaks

• You are using a matrix peak to calibrate

NOTE: Low mass gate is automatically disabled when you

change the instrument mode to PSD.

Low Mass Gate

spike

When Low Mass Gate is turned on, a characteristic spike may

occur in the spectrum (Figure 5-20). This spike is typically only

seen in Reflector mode.

NOTE: Systems with high current detectors (Voyager-DE

systems with serial number 1128 and later; Voyager-DE

PRO systems with serial number 6007 and later;

Voyager-DE STR systems with serial numbers 4112, 4113,

and 4116 and later) do not typically exhibit this spike. Older

systems (with a dual channel plate detector in Linear mode)

may exhibit a 50 to 1,000 mV spike.

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