Voyager DP-263-S Guía de usuario descargar pdf (Pagina 400)

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Chapter 6 Acquiring Mass Spectra

6-88 PerSeptive Biosystems

If standard signal suppresses unknown signal, you have two

options for analysis:

• Perform internal calibration using separate spots—

Spot standard and sample as close to each other as

possible. Create a search pattern file that analyzes both

spots. Create an instrument settings file that generates a

single spectrum and specify internal calibration in

Sequence Control. See “Creating a search pattern file for

internal calibration on separate spots” on page 6-88.

NOTE: To use this strategy, make sure standard and

sample are compatible with the laser intensity and

acceptance criteria in the instrument settings, and that

the standard and sample peaks do not overlap.

• Perform close external calibration—Spot standard and

sample as close to each other as possible and perform

close external calibration as described in Section 6.7.7.3,

Performing Close External Calibration.

Creating a search

pattern file for

internal

calibration on

separate spots

To create a search pattern file for internal calibration on

separate spots:

1. Spot sample and standard as close to each other as

possible (within 1 mm) (see Figure 6-25 on page 6-85).

NOTE: For optimum mass accuracy, calibration

standards must be as close to unknowns as possible.

An internal standard spot within 1 mm of a sample spot

can yield mass accuracy close to the expected mass

accuracy for internal standard calibration on your

system.

NOTE: Use plates without laser-etched sample

positions if you spot standard and sample as shown in

Figure 6-25 on page 6-85.

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