Voyager DP-263-S Guía de usuario descargar pdf (Pagina 146)

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Chapter 3 Preparing Samples

3-16 PerSeptive Biosystems

You can load polymer samples on sample plates in two ways:

• Thin layer polymer method (yields even response,

but provides adequate sample response for only

10 laser shots)—Load 0.3 µl sample/matrix solution in

one sample position.

• Thick layer polymer method (yields less even

response, but provides adequate sample response

for 50 to 100 laser shots)—Load 2 to 3 µl sample/matrix

solution in one sample position by placing the pipet tip in

the sample position and slowly expelling while keeping

the tip in contact with the sample plate.

NOTE: In general you should not touch the pipette tip

to the sample plate surface. During the thick layer

method it is necessary to touch the tip to the sample

plate when using THF as the solvent, in order to slowly

expel the sample onto the sample plate.

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