Voyager DP-263-S Guía de usuario descargar pdf (Pagina 151)

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101

Preparing Samples

Voyager

™

Biospectrometry

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Workstation User’s Guide 3-21

3.1.5.2 Drop Dialysis (Floating Membrane)

When to use

Use this technique on polar compounds when you know

contaminants are of low molecular weight. This technique

works well for DNA and polar proteins such as glycoproteins.

What you need

For drop dialysis, you need a membrane:

• With a pore size of 0.025 µm or smaller

• That does not adhere to your sample

Procedure

To perform drop dialysis:

1. Fill a small container (for example, a pipet tip box) with

about an inch of deionized water.

2. Place the container on a stable surface.

3. Use forceps to place the membrane in the water with the

appropriate side up (refer to manufacturer’s information).

Do not use your fingers because you can contaminate the

membrane with oil and salt.

4. Place about 10 parts of sample (for example, 10 µl) in the

center of the membrane.

5. Place 1 part of pure acetonitrile (for example, 1 µl) on top

of the sample spot to increase surface area. Do not

exceed a 10 percent concentration of organic. It may

dissolve the membrane.

6. Cover the container to prevent drying and allow it to sit for

30 minutes.

7. Note the size of the sample/organic spot, then let it sit for

30 more minutes.

The size of the sample spot increases as dialysis is

occurring. This can be used to check for completion.

8. Observe the size of the sample/organic spot. If the size of

the spot is larger than the first time you checked it, allow

the sample to sit for 15 more minutes. Observe the size of

the sample/organic spot again.

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Voyager DP-263-S Guía de usuario Pagina 151

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