Voyager DP-263-S Guía de usuario descargar pdf (Pagina 163)

100

101

Loading Samples on Sample Plates

Voyager

™

Biospectrometry

™

Workstation User’s Guide 3-33

3.2.3 Loading Samples

(Thin Layer Application)

When to use

Use the thin layer application technique

for increased

sensitivity when analyzing peptides with a concentration

<0.1 pmol/ µl.

Loading matrix

and samples

To load matrix and samples:

1. Dispense 0.5 µl of matrix prepared for thin layer

application on the sample plate to form a thin layer. It

will dry in seconds.

For information, see “CHCA for thin layer application”

on page 3-9.

2. Load 0.5 to 1 µl of the sample in each sample position

onto the thin matrix layer applied in step 1.

3. Dry the plate with a heat gun.

4. After drying, load 2 to 3 µl of double-distilled water on

top of each dried sample to wash the sample.

5. Wait 5 seconds, and blow off the water droplet with

compressed air.

6. Repeat step 4 and step 5 two more times.

NOTE: During acquisition, use the “surfing method”

(moving the sample plate after every 5 to 10 shots, to a new

position on the sample).

1. Shevchenko, A., M. Wilm, O. Vorm, M. Mann,

Anal. Chem.,

1996, 68,

850–858.

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