Voyager DP-263-S Guía de usuario descargar pdf (Pagina 156)

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Chapter 3 Preparing Samples

3-26 PerSeptive Biosystems

3.1.6 Mixing Sample and Matrix

(Dried Droplet Application)

When to use

Use dried droplet application if you are analyzing samples with

a concentration >0.01 pmol/µl.

If you are analyzing samples with a concentration

<0.01 pmol/µl, use the thin layer application technique

described in Section 3.2.3, Loading Samples

(Thin Layer Application).

Premixing sample

and matrix

Mix sample and matrix in microcentrifuge tubes before

applying sample to the sample plate when you are:

• Working with concentrated samples with a high salt

concentration and need to make dilutions

• Preparing many samples

• Analyzing non-polar samples and matrix prepared in high

organic concentration that evaporates rapidly

For peptides and proteins, mix 1 µl of sample (concentration of

0.1 to 100 pmol/µl) and 9 µl of matrix in a microcentrifuge tube,

for a final concentration of 0.1 to 10 pmol/µl. Mix well on a

vortex mixer or shake by hand.

For other compounds, refer to “Matrix Information” on

page 3-6 to determine the volume of sample and matrix to mix

to yield the necessary final sample concentration.

NOTE: If sample concentration is too high, the sample

signal may be suppressed. If sample concentration is too

low, sample signal may not be present.

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