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Voyager DP-263-S manuales

Manuales del propietario y guías del usuario para Sistemas de intercomunicación de la puerta Voyager DP-263-S.
Ofrecemos 3 manuales en pdf Voyager DP-263-S para descargar gratis por tipos de documentos: Guía de usuario, Manual de usuario


Voyager DP-263-S Guía de usuario (612 paginas)


Marca: Voyager | Categoria: Sistemas de intercomunicación de la puerta | Tamaño: 4.15 MB |

 

Tabla de contenidos

Biospectrometry

1

Workstation

1

Chapter 3 Preparing Samples

5

...................... 5-39

7

...................... 6-60

9

Chapter 7 PSD Analysis

10

Table of Contents

11

Instrument Safety

13

Safety symbols

15

Electrical Symbols

15

Non-electrical

16

Symboles non

18

Workstation User’s Guide xix

19

Workstation User’s Guide xxi

21

Safety and EMC

23

Sécurité

24

Laser Safety

25

How to Use This Guide

27

1 Introducing the

31

Workstations

31

1.1 Voyager

32

-DE and

32

Voyager-DE PRO

32

System Overview

32

Features

34

1.2 Voyager-DE STR

35

1.3 MALDI-TOF MS

37

Technology Overview

37

Ionization

38

Workstation User’s Guide 1-9

39

Advantages of

40

MALDI-TOF

40

1.4 Voyager-DE

41

Technology

41

1-12 PerSeptive Biosystems

42

Extraction (Linear Mode)

43

Benefits of

44

Extraction

44

Velocity focusing

45

—Detection

46

1.5.1 System Components

47

PB100465

47

PB100466

48

1.5.2 Mass Spectrometer

50

Parts of the mass

52

1.5.3 Vacuum System

54

Vacuum pumps

56

Vacuum gauges

56

1.5.4 Vacuum Gauge Panel

59

1-30 PerSeptive Biosystems

60

1.5.5 Computer Components

61

1.6.1 System Components

62

1.6.2 Mass Spectrometer

64

4113, and 4116 and later

66

1.6.3 Vacuum System

68

(Top View)

69

1.6.4 Vacuum Gauge Panel

70

1.6.5 Front Panel Indicators

72

1.6.6 Computer Components

73

Software

74

1.7 Software Overview

75

1-46 PerSeptive Biosystems

76

(Data Explorer)

78

2 Installing the

79

Voyager™

79

Biospectrometry™

79

2.2.1 Voyager-DE and

80

Voyager-DE PRO Workstations

80

Selecting the Site

81

Workstation User’s Guide 2-3

81

PB100507

82

2-6 PerSeptive Biosystems

84

2-8 PerSeptive Biosystems

86

PB100776

87

PB100784

88

2.3.2 Connecting the

89

2.3.3 Connecting the

90

2.3.4 Connecting the

91

LSA1000 LeCroy Digitizer

91

2-14 PerSeptive Biosystems

92

PB100770

93

2-16 PerSeptive Biosystems

94

NOTE: There are two 15-pin

96

2.4 Connecting the

97

Voyager-DE STR Workstation

97

Video monitors

100

Biospectrometry

101

2.5 Installing Software

102

Installing Software

103

Accessing the

104

2.5.2 Starting the Software

105

2-28 PerSeptive Biosystems

106

2.5.3 Exiting the Software

107

2.6 Hardware Configuration

108

Hardware Configuration

109

2-32 PerSeptive Biosystems

110

High voltage

111

Timed ion

112

Instrument

113

2-36 PerSeptive Biosystems

114

Digitizer

116

In this section

117

Overview

117

When to align

117

2-40 PerSeptive Biosystems

118

Alignment

119

2-42 PerSeptive Biosystems

120

Using the control

121

Before aligning

121

Aligning

121

2-44 PerSeptive Biosystems

122

Aligning the Sample Plate

123

2.8 Startup and Shutdown

124

Startup and Shutdown

125

2-48 PerSeptive Biosystems

126

Powering down

127

2.9 Checking System Status

128

Checking System Status

129

2-52 PerSeptive Biosystems

130

3 Preparing Samples

131

3.1 Preparing Samples

132

3.1.1 Selecting a Matrix

133

3.1.2 Preparing Matrix

134

Preparing Samples

135

Workstation User’s Guide 3-5

135

3.1.3 Matrix Information

136

Workstation User’s Guide 3-7

137

3-8 PerSeptive Biosystems

138

Workstation User’s Guide 3-9

139

3-10 PerSeptive Biosystems

140

DHB for neutral

142

DHB for small

143

3-14 PerSeptive Biosystems

144

Synthetic polymer

145

3-16 PerSeptive Biosystems

146

3.1.4 Preparing Sample

147

Preparing

148

Internal standards

148

3.1.5 Sample Cleanup

149

3.1.5.1 Washing

150

When to use

151

What you need

151

Procedure

151

3-22 PerSeptive Biosystems

152

3.1.5.3 Cation Exchange

153

3.1.5.4 ZipTips

154

(Dried Droplet Application)

156

Mixing sample

157

3.2.1 Overview

158

Types of sample

159

3-30 PerSeptive Biosystems

160

3.2.2 Loading Samples

161

3-32 PerSeptive Biosystems

162

3.2.3 Loading Samples

163

(Thin Layer Application)

163

PB100265

164

PB100508

165

PB100509

165

PB100511

165

PB100510

165

3.3 Cleaning Sample Plates

166

Cleaning Sample Plates

167

3-38 PerSeptive Biosystems

168

3.4 Loading Sample Plates

169

3-40 PerSeptive Biosystems

170

3.4.2 Loading Sample Plates

172

PB100278

173

65 66

173

74 75 76

173

84 85 86

173

3-44 PerSeptive Biosystems

174

PB100277

175

3-46 PerSeptive Biosystems

176

Sample Plate Types Supported

177

3-48 PerSeptive Biosystems

178

3-50 PerSeptive Biosystems

180

.PLT files

181

3.5.3 Guidelines for

182

Defining Custom Plate Types

182

3-54 PerSeptive Biosystems

184

3-56 PerSeptive Biosystems

186

3-58 PerSeptive Biosystems

188

3-60 PerSeptive Biosystems

190

3-62 PerSeptive Biosystems

192

3.5.4 Adjusting the Laser

193

3-64 PerSeptive Biosystems

194

4 Voyager

195

Instrument Control

195

Panel Basics

195

4.1 Instrument Control Panel

196

Instrument Control Panel

197

Workstation User’s Guide 4-3

197

Control pages

198

Voyager-DE STR system

198

Spectrum window

199

Status bar

199

Figure 4-2 Status Bar

199

Output window

199

Displaying

200

Manual control

200

Workstation User’s Guide 4-7

201

4.2 Using the Control Pages

202

Types of page

203

4-10 PerSeptive Biosystems

204

Using the Spectrum Window

205

4-12 PerSeptive Biosystems

206

4.3.2 Zooming on Traces

207

4-14 PerSeptive Biosystems

208

Figure 4-5 Adding Traces

209

Removing traces

209

4.3.4 Annotating Traces

210

4-18 PerSeptive Biosystems

212

5. Click Print

213

4-20 PerSeptive Biosystems

214

4.4 Customizing the

215

4-22 PerSeptive Biosystems

216

Instrument Menu Commands

218

Controlling the Workstation

219

4-26 PerSeptive Biosystems

220

Selecting Sample Position

221

4-28 PerSeptive Biosystems

222

4-30 PerSeptive Biosystems

224

4.6 Sequence Control Panel

226

4.7 How the Instrument

227

Control Panels Interact

227

4-34 PerSeptive Biosystems

228

Sequence Control Panel

229

4-36 PerSeptive Biosystems

230

5 Optimizing

231

Instrument Settings

231

5.1 Loading, Modifying, and

232

Saving Instrument Settings

232

5.1.2 Standard Instrument

233

List of .BIC files

234

Workstation User’s Guide 5-5

235

5-6 PerSeptive Biosystems

236

5.1.3 Opening and Viewing

237

5.1.4 Modifying an

238

Modifying for

239

Manual Control

239

5-10 PerSeptive Biosystems

240

5.1.5 Saving and Printing

241

Saving .BIC files

242

Printing

242

Files to “Read-Only” Status

243

5.2 Instrument Settings

244

Parameter Descriptions

244

5-16 PerSeptive Biosystems

246

5-18 PerSeptive Biosystems

248

5-20 PerSeptive Biosystems

250

Data Explorer Software

251

User’s Guide

251

Matrix influence

252

J. Am. Soc. Mass. Spectrom.

252

Modifying the

253

Instrument Mode Tab Displayed

254

Voyager-DE system

256

5-28 PerSeptive Biosystems

258

Advanced

259

Advanced Tab Displayed

259

5-30 PerSeptive Biosystems

260

5-32 PerSeptive Biosystems

262

5-34 PerSeptive Biosystems

264

5-36 PerSeptive Biosystems

266

5-38 PerSeptive Biosystems

268

5.3.1 Summary of Parameters

269

5-40 PerSeptive Biosystems

270

Potential gradient

272

Maximum allowed

273

Adjusting

273

5-44 PerSeptive Biosystems

274

Relationship to

275

Grid Voltage%

275

Figure 5-10 Beam Guide Wire

276

All models

276

Linear mode

276

Reflector mode

277

PSD mode

277

Horizontal

277

5-48 PerSeptive Biosystems

278

Effects of

279

Resolution and Data Quality

279

• Input Bandwidth

280

Suggested settings

281

5-52 PerSeptive Biosystems

282

Bandwidth setting

283

5.4 Optimizing Instrument

284

Settings Parameters

284

5.4.1 Optimization Strategy

285

Strategy

286

Laser intensity

287

Adjusting laser

288

General strategy

288

Signal saturation

289

5-60 PerSeptive Biosystems

290

5.4.3 Optimizing Resolution

291

5.4.3.1 Overview

292

5-64 PerSeptive Biosystems

294

Delayed Extraction Mode

295

For more

296

• Optimizing Delay Time

297

5-68 PerSeptive Biosystems

298

Voltage% Values

299

Time and Grid Voltage% Values

299

5-70 PerSeptive Biosystems

300

5-72 PerSeptive Biosystems

302

5-74 PerSeptive Biosystems

304

5.4.4.1 Overview

306

5-78 PerSeptive Biosystems

308

5-80 PerSeptive Biosystems

310

5-82 PerSeptive Biosystems

312

6 Acquiring

313

Mass Spectra

313

6.1 Before You Begin

314

Before You Begin

315

Workstation User’s Guide 6-3

315

6-4 PerSeptive Biosystems

316

Workstation User’s Guide 6-5

317

6-6 PerSeptive Biosystems

318

Types of

319

When to calibrate

320

Workstation User’s Guide 6-9

321

Data Explorer

322

Software User’s Guide

322

6.2.1 Manually Acquiring

323

6-12 PerSeptive Biosystems

324

6-14 PerSeptive Biosystems

326

6-16 PerSeptive Biosystems

328

6-18 PerSeptive Biosystems

330

6.3 Obtaining Good Spectra

332

Lower laser power producing:

333

6.3.2 Laser Intensity

335

6.3.3 Parameters Affecting

336

6.4 Making Accurate

337

Mass Measurements

337

6-26 PerSeptive Biosystems

338

6.5.1 Detecting

339

6-28 PerSeptive Biosystems

340

Height for most applications

341

6-30 PerSeptive Biosystems

342

____ ____

343

6-32 PerSeptive Biosystems

344

(± AMU)

345

5. Click OK

345

6.6.1 Before Acquiring in

346

Automatic Control Mode

346

Control mode

348

Spectrum

350

6-40 PerSeptive Biosystems

352

Continued

353

Saving the

354

6.6.4 Search Pattern Files

356

0,0 1270,0-1270,0

357

Default search

358

6-48 PerSeptive Biosystems

360

Acquisition in Automatic Mode

361

6-50 PerSeptive Biosystems

362

6-52 PerSeptive Biosystems

364

Control dialog box is used

365

6-54 PerSeptive Biosystems

366

6.6.6 Process that Occurs

368

Accumulating All Spectra

369

Accumulating Passing Spectra

370

6.7 Acquiring Spectra from

372

Macros, and Calibration

374

How file types

375

6-64 PerSeptive Biosystems

376

6-66 PerSeptive Biosystems

378

6.7.3 Creating a Sequence

379

6-68 PerSeptive Biosystems

380

6.7.3.2 Creating a Run List

381

View menu

382

6-72 PerSeptive Biosystems

384

6-74 PerSeptive Biosystems

386

6-76 PerSeptive Biosystems

388

6.7.5 Running a Sequence

390

6-80 PerSeptive Biosystems

392

Checking status

393

Control Panel

393

6.7.7 Automatic Calibration

394

During a Sequence Run

394

Optimum Mass Accuracy

396

6-86 PerSeptive Biosystems

398

6.7.7.4 Internal Standard

399

Calibration Considerations

399

6-88 PerSeptive Biosystems

400

Using Separate Spots

401

Using Workbook

402

Showing or hiding

403

Arranging

403

6-92 PerSeptive Biosystems

404

7 PSD Analysis

405

7.1 Overview of PSD Analysis

406

Overview of PSD Analysis

407

Workstation User’s Guide 7-3

407

Reflector

408

Immonium ions

409

J. Am. Soc. Mass Spectrom.

409

Chapter 7 PSD Analysis

410

7-6 PerSeptive Biosystems

410

7.1.3 PSD Data Files

411

PSD calibration

412

Default PSD

412

Workstation User’s Guide 7-9

413

Configuration Dialog Box

414

7.2 Enhancing

415

Fragmentation with CID

415

Benefits

416

Gas requirements

417

Meth. Enzymol

417

7-14 PerSeptive Biosystems

418

7-16 PerSeptive Biosystems

420

7.3 Acquiring PSD Data

421

Settings (.BIC) Files

421

Differences from

422

Generating the

423

7-20 PerSeptive Biosystems

424

7.3.2 Determining the

425

7-22 PerSeptive Biosystems

426

7-24 PerSeptive Biosystems

428

7-26 PerSeptive Biosystems

430

7-28 PerSeptive Biosystems

432

7-30 PerSeptive Biosystems

434

7-32 PerSeptive Biosystems

436

Changing

438

7.4 Exploring PSD Mode

440

7.4.1 Observing the

441

Effects of Laser Intensity

441

7-38 PerSeptive Biosystems

442

Exploring PSD Mode

443

7.4.2 Observing the Effects

444

7-42 PerSeptive Biosystems

446

Figure 7-14 Ion Focusing

448

7-46 PerSeptive Biosystems

450

7.4.4 Summary

451

7.5 Viewing PSD Data

452

8 Maintenance and

453

Troubleshooting

453

8.1.1 Maintenance Schedule

454

8.1.2 Hardware Maintenance

455

Changing fuses

456

Figure 8-1 Changing Fuses

457

(5 x 20 mm)

458

8.2 Troubleshooting

459

8-8 PerSeptive Biosystems

460

Workstation User’s Guide 8-9

461

NOTE: Input Bandwidth is not

462

NOTE: Data acquired using

463

8-12 PerSeptive Biosystems

464

(continued on next page)

465

(continued)

466

NOTE: Increasing the laser

468

8-18 PerSeptive Biosystems

470

8-20 PerSeptive Biosystems

472

Checking the

473

Windows NT

473

Event Log

473

8-22 PerSeptive Biosystems

474

8-24 PerSeptive Biosystems

476

8-26 PerSeptive Biosystems

478

Appendix

479

Appendix A Specifications

480

Voyager-DE Specifications

481

Workstation User’s Guide A-3

481

Specifications

482

Voyager-DE PRO Specifications

483

Workstation User’s Guide A-5

483

Voyager-DE STR Specifications

485

Workstation User’s Guide A-7

485

Workstation User’s Guide A-9

487

B.1 Limited Product Warranty

490

Workstation User’s Guide B-3

491

B-4 PerSeptive Biosystems

492

B.2 Damages, Claims, Returns

493

B.3 Spare Parts

494

Sample plates

495

Membrane, Gels V700698

496

Description Part Number

496

Figure C-2

498

(DHBs) Matrix Spectrum

499

Appendix C Matrices

500

C-4 PerSeptive Biosystems

500

Workstation User’s Guide C-5

501

if sample

502

Workstation User’s Guide C-7

503

PB100253

504

Workstation User’s Guide C-9

505

C-10 PerSeptive Biosystems

506

C-12 PerSeptive Biosystems

508

Sample Log

510

Appendix D Log Sheets

516

Elite, linear

518

F.1 Calibration Compounds

520

Calibration Compounds

521

Workstation User’s Guide F-3

521

F.2 Conversion of Mass to

522

Time for Typical Standards

522

Workstation User’s Guide F-5

523

F.3 Theoretical

524

Cleavages for Angiotensin

524

F.4 Observed

525

PSD Fragments in Angiotensin

525

F-8 PerSeptive Biosystems

526

Maintenance Log

528

H.1 Optimizing a Continuous

530

(.BIC) Setting

530

Workstation User’s Guide H-3

531

(.BIC) Files

532

Optimization

533

Optimizing

533

Extraction Mode

534

Continuous Extraction Mode

535

Ratio, and Laser Threshold

536

Workstation User’s Guide H-9

537

H-10 PerSeptive Biosystems

538

H.2.2.1 Overview

540

H-14 PerSeptive Biosystems

542

H-16 PerSeptive Biosystems

544

H.2.3 Checking Resolution

545

H-18 PerSeptive Biosystems

546

H-20 PerSeptive Biosystems

548

H-22 PerSeptive Biosystems

550

Oscilloscope

554

I.1 Guidelines for Acquiring

555

I.2 Scaling

556

Workstation User’s Guide I-5

557

I-6 PerSeptive Biosystems

558

I.3 Using the Control Stick

559

I-8 PerSeptive Biosystems

560

Bibliography

561

Delayed Extraction

562

MALDI Applications

562

MS/MS Interpretation

563

Peptide Applications

564

DNA Applications

564

Glossary

567

Numerics

573

Index-2 PerSeptive Biosystems

574

Index-4 PerSeptive Biosystems

576

Index-6 PerSeptive Biosystems

578

Index-8 PerSeptive Biosystems

580

Vertical Scale 5-28

583

Vertical Scale 5-28, 5-50

594

Voyager DP-263-S Manual de usuario (8 paginas)


Marca: Voyager | Categoria: Sistemas de intercomunicación de la puerta | Tamaño: 2.51 MB |

 

Tabla de contenidos

Comprehensive

1

USB 2.0 and USB 3.0

1

Protocol Verification

1

6th Generation

3

Analysis Software

3

Raw Debugging Power

4

Intelligent Triggering

4

Real Time Filtering

4

Error and Event Reporting

4

VOYAGER EXERCISER OPTION

5

Find The Issues Fast

7

Specifications

8

Ordering Information

8

Voyager DP-263-S Manual de usuario (1 paginas)


Marca: Voyager | Categoria: Sistemas de intercomunicación de la puerta | Tamaño: 0.37 MB |

 

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